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Laboratory methods and calculations used when working with research peptides in vitro.
What a lot number is in the regulations the convention comes from, how lot and batch differ, what records have to sit behind the number, how a retest date differs from an expiry date, and what a lot number cannot prove on its own.
What a systematic review is, what a meta-analysis adds, why a paper titled "systematic review" sometimes isn't one, and what the published reviews of BPC-157, GHK-Cu, TB-500 and Cerebrolysin actually concluded.
Analytical method validation explained: specificity, accuracy, precision, LOD and system suitability under ICH Q2(R2), and what they mean for purity.
COA vs SDS vs spec sheet: a COA reports one lot, an SDS covers hazards under WHMIS and a spec sheet defines the product. What each can and can't prove.
Peptide lab safety under WHMIS 2015: hazard information, PPE, weighing powders, spills, workplace labels and chemical waste disposal in Canadian labs.
HPLC vs UPLC: smaller particles and higher pressure give sharper peaks and faster runs. What that changes for peptide purity results, and what it doesn't.
Peptide molecular weight explained: calculating it from the sequence, end groups and disulfides, why salt forms weigh more, and how to check a certificate.
What a scrambled peptide control tests, how to design one, when alanine scans or inactive analogues work better, and the pitfalls that undermine controls.
Amino acid analysis explained: acid hydrolysis, derivatization and quantitation, which residues are lost, and how AAA compares with CHN, qNMR and UV.
What LC-MS is, how electrospray turns a peptide into multiply charged ions, what a mass match does and does not prove, and the three things on a certificate that LC-MS cannot answer.
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