NAD+

Description

NAD+ (nicotinamide adenine dinucleotide) is one of the most widely used coenzymes in cell biology and biochemistry, and this listing supplies it as a research-grade reagent for laboratories working with research peptides and compounds in Canada. Each vial contains 500 mg of lyophilized material, sealed and ready for reconstitution in a solvent appropriate to your assay. It is intended for in-vitro and analytical work only — not for human or veterinary use.

Overview

NAD+ is the oxidized form of the pyridine dinucleotide redox pair NAD+/NADH, the electron carrier at the centre of glycolysis, the TCA cycle and oxidative phosphorylation, and the substrate consumed by sirtuins, PARPs and CD38 in non-redox enzymology. That makes it a routine reagent in enzyme kinetics, dehydrogenase-coupled assays, mitochondrial and metabolic studies, and cell-free systems where cofactor supply has to be controlled rather than assumed. This product is supplied as a lyophilized powder in a sealed vial at 500 mg, available as a single vial or a box of ten for labs running extended or parallel protocols. Reconstitution volume and solvent are left to the researcher, so the same vial can be prepared as a concentrated stock for aliquoting or made up fresh at working concentration.

What it is known for

Buyers choose this reagent for the paperwork behind it as much as the material itself. Every batch goes to Janoshik, an independent analytical laboratory, for HPLC purity and mass spectrometry identity confirmation, and the resulting certificate of analysis is matched to the batch you receive rather than to a representative sample from some earlier production run. For anyone whose results have to survive review, that traceable chain from vial to COA is the difference between a reagent you can cite and one you have to qualify in-house first.

Key features

• Lyophilized powder format — Freeze-dried material is more stable in transit and storage than a shipped solution, and it lets you choose the solvent and concentration that suit your protocol instead of working around a fixed formulation.
• 500 mg fill per vial — Enough material for repeat runs, dose-response series or multi-plate assays from a single reconstitution, which reduces vial-to-vial variability within an experiment.
• Third-party HPLC purity testing — An outside lab quantifies purity rather than the supplier self-reporting it, so you know what fraction of the mass is the compound before you calculate a working concentration.
• Mass spectrometry identity confirmation — Confirms the molecular identity of the material, ruling out mislabeling or substitution — a basic control before any result built on the reagent is trusted.
• Batch-matched certificate of analysis — The COA corresponds to the specific lot in your hands, which is what documentation and audit trails actually require.
• Box-of-ten option — Ten vials from the same purchase keeps a longer study on consistent material and reduces the number of separate lots you have to reconcile in your records.
• Cold storage and tracked Canadian shipping — Held cold before dispatch and shipped domestically with tracking, so the material spends less time in transit and you can see where it is.
• Discreet packaging — Plain outer packaging keeps deliveries to shared labs, campus mailrooms and receiving desks unremarkable.

Benefits

• Independent purity and identity data on the exact lot you receive, so reagent quality is documented rather than assumed
• Freedom to set your own solvent, concentration and aliquot scheme from a single lyophilized vial
• Domestic shipping within Canada with tracking, avoiding cross-border customs delays for temperature-sensitive material
• Consistent supply across a study when ordered as a box of ten
• Documentation that stands up when a protocol, grant report or publication asks where the reagent came from

Specifications

Typical applications

• Enzyme kinetics and dehydrogenase-coupled assays requiring a defined NAD+ supply
• Studies of NAD+-consuming enzymes such as sirtuins, PARPs and CD38 in cell-free or lysate systems
• Cellular metabolism and mitochondrial function research in vitro
• Redox balance and NAD+/NADH ratio measurement work
• Analytical method development, standard curves and instrument calibration
• Preparation of stock solutions for aliquoting across a multi-run study

Important information

• Research use only. This product is not a drug, supplement, food or cosmetic, is not intended to diagnose, treat, cure or prevent any disease, and is not for human or veterinary use, ingestion or administration.
• Reconstitute in a solvent suitable for your assay; solvent choice, working concentration and buffer compatibility are the researcher’s responsibility.
• Keep the vial sealed and cold until use, and minimise repeated freeze-thaw cycles of reconstituted stock by aliquoting.
• NAD+ in solution is sensitive to pH and elevated temperature — prepare working solutions close to the time of use where your protocol allows.
• Request the batch-matched certificate of analysis and file it with your lot records before beginning work.
• Handle with standard laboratory PPE and dispose of according to your institution’s chemical waste procedures.

Common questions

What form does the product arrive in?

A sealed vial of lyophilized (freeze-dried) NAD+ powder, 500 mg per vial. No solvent is included, so you control the reconstitution.

How is purity verified?

By an independent analytical laboratory, Janoshik, using HPLC for purity and mass spectrometry for identity. The certificate of analysis is matched to the batch you receive, not to an earlier production run.

Can I get the certificate of analysis for my specific vial?

Yes. A batch-matched COA is available for the lot supplied — request it with your order and keep it with your lot documentation.

What is the difference between the single vial and the box of ten?

The material is the same. The box of ten suits longer or parallel studies where you want to stay on consistent supply and reduce the number of separate lots recorded in your notes.

Where does it ship from and how long does it take?

It ships from within Canada with nationwide tracking, in discreet packaging. Transit time depends on destination and the service selected at checkout.

Is this suitable for human use?

No. It is supplied strictly for in-vitro laboratory research and is not a drug, supplement or food. It is not intended for human or veterinary administration in any form.

In summary

A 500 mg lyophilized vial of NAD+ with independent HPLC purity and MS identity data behind it, a certificate of analysis tied to your batch, and tracked shipping from within Canada. For laboratories that need a redox cofactor they can document as well as use, in single vials or boxes of ten. Research use only.