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The Research-Peptide Glossary: Key Terms Every Lab Should Know

New to research peptides? This glossary defines the terms you’ll meet on listings and Certificates of Analysis โ€” in plain English.

Quality & testing

Certificate of Analysis (COA) โ€” A lab document reporting the measured quality of a specific batch: purity, identity, and other parameters. Should be batch-specific.

HPLC (High-Performance Liquid Chromatography) โ€” A method that separates a sample to measure purity, shown as peaks on a chromatogram.

Mass spectrometry (MS) โ€” Measures molecular mass to confirm identity (the molecule is what the label says).

Purity โ€” The percentage of the target peptide among peptide-related species, by HPLC. A common high standard is โ‰ฅ99%.

Net peptide content โ€” How much of the vial’s mass is actually peptide (vs. salt and water); often 80โ€“95%.

Chromatogram โ€” The graph from HPLC; one tall, sharp peak signals high purity.

Third-party / accredited lab โ€” An independent, audited lab whose results are more credible than in-house figures.

Composition & form

Lyophilized โ€” Freeze-dried into a stable powder by removing most water.

Counter-ion โ€” The salt form a peptide ships in, usually acetate or TFA (trifluoroacetate); affects net peptide content.

Karl Fischer (KF) โ€” A titration that measures residual water in the powder.

Endotoxin โ€” Bacterial contaminants sometimes reported on a COA; lower is better.

Sequence โ€” The ordered chain of amino acids that defines the peptide.

Molecular weight (MW) โ€” The mass of one mole of the compound (g/mol); needed to convert to molar units.

Concentration math (in-vitro)

Molarity (M) โ€” Moles per litre; counts molecules. mM = millimolar, ยตM = micromolar.

Stock solution โ€” A concentrated solution you later dilute.

Dilution (Cโ‚Vโ‚ = Cโ‚‚Vโ‚‚) โ€” The equation for making a weaker solution from a stronger one.

mg/mL โ€” Milligrams of solute per millilitre of solvent; a mass-per-volume concentration.

Logistics

Batch / lot number โ€” A code identifying a production run; match it to your COA and vial.

Cold-chain โ€” Temperature-controlled handling/shipping for products that need it.

Research use only (RUO) โ€” Sold strictly for laboratory research; not for human or veterinary use.

Key takeaways

  • HPLC = purity, MS = identity, COA = the batch report tying it together.
  • Lyophilized powder is stable when kept cold, dark and dry.
  • Net peptide content and counter-ion explain what’s really in the vial.

FAQ

What does “lyophilized” mean in one sentence? Freeze-dried into a dry, stable powder by removing water.

What’s the difference between mM and ยตM? 1 mM = 1000 ยตM. Both are molar concentrations; mM is a thousand times stronger than ยตM.

What does RUO mean? Research use only โ€” the product is for laboratory research, not for human or veterinary use.

Browse verified research peptides

Every batch we ship is independently tested by Janoshik Analytical โ€” HPLC for purity, mass spectrometry for identity โ€” with a batch-matched Certificate of Analysis you can verify yourself. Browse the catalog โ†’ ยท See the latest COAs โ†’


*For laboratory and research use only. Not for human or veterinary use, consumption, or administration. Nothing in this article is medical advice or a claim that any compound treats, cures, or prevents any condition.*

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